The phytopathogenic fungus Fusarium graminearum produces mycotoxins, predominantely the trichothecene Deoxinevalenol (DON). To investigate the strategies of detoxification of the fungus, we analysed the gene expression of a set of putative transporter genes upon application of azole fungicides, trifloxistrobin and DON. Semi-quantitative and realtime-PCR is performed to screen for differential expressed genes (Fig.1).      

 

 

Candidate genes with a transcriptional response to the treatments are further analysed by realtime-PCR and will be functionally characterized.